Abstract
bioactivity and the inhibitory activity of the endophytic fungi were evaluated against various phytopathogenic fungi: Fusarium
oxysporum f. sp. lycopersici (Sacc.) Snyder y Hansen, F. oxysporum Schlect. f.sp. cubense (E.F. Smith) Snyd. & Hans, F. moniliforme (Sheldon), Alternaria alternata (Fr.) Keissl, Geotrichum sp, Phoma sp. and Cladosporium sp. In dual culture
assays, endophytic strains of Penicillium, Aspergillus, Fusarium and Chaetomium were found to be active against some of
the targeted microorganisms. Four active extracts were fractionised using VLC (vacuum liquid chromatography) and two ethyl acetate and ethanolic extracts obtained from Aspergillus oryzae isolate led to two active hexanic and ethyl acetate fractions which inhibited growth of F. moniliforme (%I = 48.02 and 55.1, respectively; at 1 mg ml?1). The smallest EC50 (0.74 mg ml?1) was observed in an extract obtained from a strain of Penicillium sp. The same endophytic strain inhibited F. oxysporum by 59.72% in VOC (volatile organic compounds) assays.
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References
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