Journal of Pharmaceutical and Biomedical Sciences

Antioxidative Effect of Graviola Aqueous Extract on the Blood of the Patients from Diabetes

Bhaumik D. Vaghela, Pooja N. Thakkar, Kinjal P. Bhadresha, Nayan K. Jain

Abstract


This study was aimed to look into the antioxidative effect of the graviola aqueous extract on the blood of the patients from diabetes. 5 ml Blood was collected from the patients of 45-50 years of age group and then equally divided into two groups non-treated (Diabetic) and treated. Oxidative tests were carried out directly from the diabetic group whereas; in the treated group 1 ml of the graviola aqueous extract was added. Various biochemical tests for example, Lipid Peroxidation (LPO), Catalase (Cat), Glutathione Peroxidase (GPx), Superoxide Dismutase (SOD) were carried out. Results were positive showing antioxidative effect of the graviola aqueous extract.

Keywords


diabetes, graviola aqueous extract, oxidative stress

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References


Hunt JV, Dean RT, Wolff SP. Hydroxyl radical production and autoxidative glycosylation. Glucose autoxidation as the cause of protein damage in the experimental glycation model of diabetes mellitus and ageing. Biochem J. 1988;256:205–212.

Jaganjac M, Tirosh O, Cohen G, Sasson S, Zarkovic N. Reactive aldehydes—second messengers of free radicals in diabetes mellitus. Free Radic Res. 2013;47:39–48.

Negre-Salvayre A, Salvayre R, Augé N, Pamplona R, Portero-Otín M. Hyperglycemia and glycation in diabetic complications. Antioxid Redox Signal. 2009;11:3071–3109.

Taylor L. Technical data report for graviola, annona muricata. Sage Press. Austin. 2002;10:1–6.

Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979;95:351–358.

Rotruk JT, Pope AL, Ganther HC, Swanson AB, Hafeman DG, Hoekstro WG. Selenium: Biochemical role as a component of glutathione peroxidase. Science. 1973;179:588–590.

Sinha AK. Colorimetric assay of catalase. Anal Biochem. 1972;47:389–94.

Kakkar P, Das B, Viswanathan PN. A modified spectrophotometric assay of superoxide dismutase. Ind J Biochem Biophys.1984;21:130–132.

Halliwell B, Chirico S. Lipid peroxidation: its mechanism, measurement, and significance. Am J Clin Nutr. 1993;57:715S–724S.

Shodehinde SA, Oboh G. Antioxidant properties of aqueous extracts of unripe Musa paradisiaca on sodium nitroprusside induced lipid peroxidation in rat pancreas in vitro, Asian Pacific. J Trop Biomed. 2013;3:449–457.

Moussa SA. Oxidative stress in diabetes mellitus, Romanian J Biophys. 2008;18:225–236.

Singh M, Shin S. Changes in erythrocyte aggregation and deformability in diabetes mellitus: a brief review. Indian J Exp Biol. 2009;47:7–15.

Varashree BS, Bhat PG. Correlation of lipid peroxidation with glycated haemoglobin levels in diabetes mellitus. Online J Health and Allied Sci. 2011;10:1–4.

Calabrese V, Cornelius C, Leso V, Trovato-Salinaro A, Ventimiglia B, Cavallaro M, et al. Oxidative stress, glutathione status, sirtuin and cellular stress response in type 2 diabetes. Biochimica et Biophysica Acta. 2012;1822:729–736.

Dinçer Y, Akçay T, Alademir Z, Ilkova H. Assessment of DNA base oxidation and glutathione level in patients with type 2 diabetes. Mutat Res. 2002;505:75–81.

Shukla K, Dikshit P, Tyagi MK, Shukla R, Gambhir JK. Ameliorative effect of Withania coagulans on dyslipidemia and oxidative stress in nicotinamide-streptozotocin induced diabetes mellitus. Food and Chem Toxicol. 2012;50:3595–3599.

Chelikani P, Fita I, Loewen PC. Diversity of structures and properties among catalases. Cell Mol Life Sci. 2004;61:192–208.

Góth L, Eaton JW. Hereditary catalase deficiencies and increased risk of diabetes. Lancet. 2000;356;1820–1821.

Baskar R, Rajeswari V, Kumar TS. In vitro antioxidant studies in leaves of annona species. Indian J Exp Biol. 2007;45:480–485.

Vaghela BD, Bhavsar D, Jain NK. Effect of Aqueous extract of Annona muricata (Graviola Tea) on the oxidative stress produced by the artificial UV source in vivo and in vitro. Int J Curr Microbiol App Sci. 2016;5:648–655.

Vijayameena C, Subhashini G, Loganayagi M, Ramesh B. Phytochemical screening and assessment of antibacterial activity for the bioactive compounds in Annona muricata. Int J Curr Microbiol Appl Sci. 2013;2:1–8.


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