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Identification of Strain H41-55 and Chemical Constituents from its Fermentation Broth

Xiansheng Li, Birun Lin, Guangxiong Zhou

Abstract


The strain H41-55 was identified as Streptomyces antibioticus according to morphological, cultural, and physiological and biochemical characteristics in combination with 16S rDNA sequence and phylogenetic tree analysis of the strain, and the 16S rDNA accession number in Genbank was FJ702547. Five steroid and two actinomycin compounds, named ergosta-7,22-diene-3b,5a,6b-triol (1), ergosta-8 (14),22E-diene-3b,5a,6b,7a-tetraol (2), 3b,5a,9a-trihydroxy-(22E,24R)-ergosta-7,22-diene-6-one (3), ergosta-4,6,8 (14),22-tetraen-3-one (4) and (22E,24R )-ergosta-4,7,22-trien-3-one (5), actinomycin D (6), actinomycin X2 (7), were isolated from the fermentation broth of strain H41-55. The structures of the steroid were determined by spectroscopic methods including NMR and MS as well as comparison with the data of literatures

Keywords


streptomyces antibioticus, identification, steroid, fermentation

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References


Lam KS. Discovery of novel metabolites from marine actinomycetes. Curr Opin Microbiol. 2006;9:245–251.

Prashith KTR, Shobha KS, Onkarappa R. Fascinating diversity and potent biological activities of actinomycete metabolites. J Pharm Res. 2010;3:250–256.

Osada H. Actinomycetes, how fascinating microorganisms. Actinomycetologica. 1998;12:85–88.

The actinomycetes classification group in the Chinese academy of sciences institute of microbiology [M]. Manual of Determinative Streptomyces. 1975.

Ding CF, Wang MJ, Qiu ZD, Xu W. Chemical composition of ethyl acetate fraction of Notoginseng Radix etRhizoma medicinal fungal. Chinese J Exp Trad Med Form. 2015;21:43–46.

Sun Y, Tian L, Huang J, Li W, Pei YH. Cytotoxic sterols from marine derived fungus Pennicillium sp. Nat Prod Res. 2002;20:381–384.

Tao MH, Yan J, Wei XY, et al. Steroidal metabolites of Fimetariell arabenhorstii, endophytic fungus from Aquilaria sinensis. J Trop Subtrop Bot. 2011;19:75–78.

Cai QX, Zhao JH, Wang JY, et al. Isolation and identification of endophytic fungus LM033 obtained from Moringa oleifera Lam and biological activity of the metabolites. Chinese J New Drugs. 2013;22:2168–2173.

Zhang W, Draeger S, Schulz B, et al. Ring B aromatic steroids from an endophytic fungus, Colletotrichum sp. Nat Prod Commun. 2009;4:1449–1454.

Sarkar S, Saha M, Roy D, Jaisankar P, Das S, Gauri Roy L, et al. Enhanced production of antimicrobial compounds by three salt-tolerant actinobacterial strains isolated from the Sundarbans in a niche-mimic bioreactor. Mar Biotechnol. 2008;10:518–526.


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