Construction and Expression of Transmembrane and Secretory Recombinant Human CD4 Extracellular Domain in HEK293F Cell

Jiao Xu; Wenjia Yu; Chun Chen; Yingsha Deng; Yun Lei; Jia Lu; Zhuoteng, Cai; Qiuling Xie; Sheng Xiong

Construction and Expression of Transmembrane and Secretory Recombinant Human CD4 Extracellular Domain in HEK293F Cell

Jiao Xu, Wenjia Yu, Chun Chen, Yingsha Deng, Yun Lei, Jia Lu, Zhuoteng, Cai, Qiuling Xie, Sheng Xiong

Abstract

Cluster of differentiation 4 (CD4) is a common receptor and signal transduction molecule expressed on CD4+ cell membrane. CD4 molecules involve in the identification of major histocompatibility complex II molecules, which plays an important role in the development and differentiation of T lymphocyte subsets and CD4+ cell antigen recognition. The extracellular domain of CD4 protein contains a lot of epitopes, which is more potent than that of antigen peptide molecules. In this study, we engineered and constructed an extracellular domain of CD4 protein (CD4ECD) and an CD4ECD-Fc fusion protein. The recombinant vectors were transfected into HEK293F cells, and then expressed and purified. Functional assay showed that the CD4ECD was successfully expressed on the cell membrane, and the CD4ECD-Fc fusion was obtained in cell supernatant.

Keywords

cluster of differentiation, major histocompatibility complex (MHC), T lymphocyte.

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