Journal of Agriculture Biotechnology

Login
Notifications
Font Size

Genetic Identification of Quantitative Trait Loci (QTL) for High Nutritional Value Traits in DHLs derived from Black Rice

M. Fazaa, G. Anis, Tahany M. Mazal, A. EL Sabagh, C. Barutçular, M. S. Islam

Abstract


In the present research, thirteen different tested genotypes were selected according to their nutritional values. Five parents and eight DHLs, these DHLs were chosen for their highest and lowest mean values from each of the four crosses to validate these markers. Four SSR markers i.e. RM 590, RM 152, RM 153 and RM 295 were used and all of them generated polymorphic bands among the different genotypes. Two alleles were detected for each marker among thirteen genotypes for each nutritional value trait. The level of polymorphism generated was good to study the diversity between the selected genotypes and SSR markers which had a relationship with nutritional value traits (high concentrations of Mn, Zn and Fe). One QTL was detected for Mn content, it was located on chromosome 10 near marker (RM 590), while (RM 152 and RM 153) are associated with QTL for high Zink concentration in grain, one major effect of QTL for zinc content was detected near the simple sequence repeats marker RM152 on chromosome 8 and one major QTL associated with high zinc in the grains, was mapped on Chromosome 5, flanked by marker RM 153 (OSR 35). On the other hand, RM 295 is used to show the polymorphism for Fe concentration and to validate for high nutritional value in tested genotypes.

Keywords


Black rice, Nutritional value, SSRs, DHLs, QTLs

Full Text:

References


Abdel-Aal, El-Sayed M., J. Christopher Young, and Iwona Rabalski, (2006). Anthocyanin composition in black, blue, pink, purple, and cereal grains. J. Agric. Food Chem 54 (13): 4696-4704.

Fazaa, M., EL-Sabagh,A., Anis, G, El-Rewainy,I., Barutçular,C., Hatipo?lu, R., Islam,MS. (2016a). The Agronomical Performances of Doubled Haploid Lines of Rice (Oryza sativa L.) Derived from Anther Culture. Journal of Agriculture Science 8(5):177-183.

Fazaa, M., EL Sabagh,A., Anis,G., EL-Rewainy,I., Barutçular,C.,Yildirim,M., Islam, M.S. (2016b).Grain Quality of Doubled Haploid Lines in Rice (Oryza sativa L.) Produced by Anther Culture. Journal of Agriculture Science 8(5):184.190.

Garcia, O.,Luisa, A., Tan,L., Fu, Y., and Sun,C. (2009). Genetic Identification of Quantitative Trait Loci for Contents of Mineral Nutrients in Rice Grain. J. Integ Plant Biol 51(1):84-92.

IRRI, (2001). Genetic enhancement for improving productivity and human health in fragile environments (Project 7). Output 5. Develop micronutrient-enriched rice to combat malnutrition in fragile environments. Int. Rice Res. Ins., DAPO Box 7777, Metro Manila, Philippines. p. 31.

IRRI, (2006). High iron and zink rice. In: Rice Science for a Better World. Int. Rice Res. Ins., DAPO Box 7777, Metro Manila, Philippines.

Murray, M.G. and W.F. Thampson, (1980). Rapid isolation of high molecular weight plant DNA. Nul. Acids Res 8:4321-4325.

RRTC, (2006). Procedure, Progress, Problem and Prospects. In: workshop on the highest productivity per unit area worldwide for rice breeders. Egypt Rice Research and Training Center.

Xiaoe Yang, Ying Feng, Zhenli He and Peter J. Stoffella, (2005). Molecular mechanisms of heavy metal hyper-accumulation and phyto-remediation. J Trace Elements Medic. Biol 18(4): 339-353.


Refbacks

  • There are currently no refbacks.